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Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
文献类型:期刊
作者:Xie, Gang[1]  Zhu, Ming[2]  Liu, Zhenjiang[3]  Zhang, Bo[4]  Shi, Mingjin[5]  Wang, Shuo[6]  
机构:[1]Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Tianjin 300457, Peoples R China.;Acad State Adm Grain, Beijing, Peoples R China.;
[2]Guizhou Entry Exit Inspect & Quarantine Bur Peopl, Guiyang, Guizhou, Peoples R China.;
[3]Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang, Peoples R China.;
[4]Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang, Peoples R China.;
[5]Kangyuan Techbio Biol Technol Co Ltd, Suqian, Peoples R China.;
[6]Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Tianjin 300457, Peoples R China.;
通讯作者:Wang, S (reprint author), Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Tianjin 300457, Peoples R China.
年:2018
期刊名称:FOOD AND AGRICULTURAL IMMUNOLOGY影响因子和分区
卷:29
期:1
页码范围:564-576
增刊:正刊
收录情况:SCI(E)(WOS:000445267400005)  
所属部门:环境与安全工程学院(环境健康与生态安全研究院 海绵城市研究院)
语言:外文
ISSN:0954-0105
人气指数:244
浏览次数:244
基金:National Key Research and Development Project of China [2016YFE0113000]; Natural Science Foundation of Jiangsu Province [BK20140543]; Natural Science Found of Hubei Province [2007ABA253]; Research project of Department of Education of Hubei Province [Q200715004]
关键词:Chemiluminescence immunoassay; magnetic particles; Aflatoxin B1; real samples
摘要:
Herein, a simple and cost-effective chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) is presented for therapid detection and analysis of Aflatoxin B1, which is a potent carcinogen found in the environment. Several physicochemical factors that potentially influence the assay performance of theproposed method were investigated in detail, including concentrations of FITC-labelled antibody, AFB1-alkaline phosphatase and magnetic particles concentration. Under the optimized ...More
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